09- Maximizing Magnetic Heat Transduction for Fast Remote-Control of Temperature -Sensitive Ion Channel TRPV1

Primary Author

Josiah Lozano

Additional Author(s)

Rohini T. Guntnur, Gabriela Romero Uribe

Faculty Mentor

Dr. Hugo Giambini

Additional Mentor(s)

Dr. Gabriela Romero Uribe

Abstract

Nearly 20 million Americans suffer from neurological diseases and roughly one million new cases are reported annually. Current methods for treating neurological disorders involve the manipulation of neural signaling via application of drugs or the use of implantable devices in the brain. While these treatments have demonstrated a certain degree of effectiveness, the associated costliness and invasiveness have led to research in alternative therapies. Recently, there has been an increasing interest in the use of heat dissipation via magnetic nanoparticles (MNPs) and alternating magnetic fields (AMFs) to manipulate neural circuitry as an alternative form of therapy1,2. The successful application of MNPs and AMFs has been demonstrated through the excitation of the receptor TRPV13 , however this technique still requires optimization to ensure local and cell-type specific manipulation of biological signals. The purpose of this project is to accomplish the following specific aims: (1) Expose cells expressing TRPV1 to AMFs and MNPs using pre-existing lab procedures, (2) record cell activity in real time to determine effectiveness of each MNP in the transduction of AMFS into heat, (3) correlate neural activity response with magnetic nanoparticles physical properties. We expect to evoke activity in cells expressing TRPV1 via the MNPs transduction of AMFs into heat and we hypothesize that cubic MNPs will be better transducers compared to spherical MNPs due to shape anisotropy. This study will serve to further refine and optimize the use of MNPs and AMFs to treat neurological diseases. Partially funded by NIH ESTEEMED 5R25EB027605

1 Christiansen MG, Senko AW, Anikeeva P. Magnetic Strategies for Nervous System Control. Annu Rev Neurosci. 2019;42:271-293. doi:10.1146/annurev-neuro-070918-050241
2 Rao S, Chen R, LaRocca AA, et al. Remotely controlled chemomagnetic modulation of targeted neural circuits. Nat Nanotechnol. 2019;14(10):967-973. doi:10.1038/s41565-019-0521-z
3 Chen R, Romero G, Christiansen MG, Mohr A, Anikeeva P. Wireless magnetothermal deep brain stimulation. Science. 2015;347(6229):1477-1480. doi:10.1126/science.1261821

Presentation

Poster

3-Min Thesis Slide

1 thought on “09- Maximizing Magnetic Heat Transduction for Fast Remote-Control of Temperature -Sensitive Ion Channel TRPV1”

  1. Great display and visuals. I do have to state that I had to rely on the reading of the materials because your video/audio was not coming through all the time-it seemed too large of a file. Anyhow, besides that technical aspect (that may not have been revealed to you) the slides were well summarized (had to download your file and play each slide with your audio. You did walk the viewer/audience in each slide and explained well.

Leave a Reply

Your email address will not be published. Required fields are marked *