Madeline Colley, Andrea Kelley, Grace Samenuk, Krista Berlin, Stephan Bach, George Perry
Alzheimer’s Disease (AD) is a a progressive, age related neurodegenerative disease characterized by the accumulation of senile plaques and neurofibrillary tau tangles. These plaques are made up of amyloid-beta peptides and may aggregate by copper mediated dityrosine cross-linking of the amyloid- β. The method used to analyze the amyloid-beta plaques and determine the proteins and peptides within the plaques is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The purpose of this experiment is to investigate the copper mediated-dityrosine cross-linking of amyloid-beta(1-42) to determine if oxidative stress alone is enough to induce fibrillation.